Technical Reference #804

804.

Identification And Characterization Of Rain; A Novel Ras-Interacting Protein With A Unique Subcellular Localization Natalia Y. Mitin‡§; Melissa B. Ramocki‡; Alfred J. Zullo‡; Channing J. Der§; Stephen F. Konieczny‡; and Elizabeth J. Taparowsky, Purdue University, The Journal of Biological Chemistry, 279(804), (2004)
Link To Paper

Abstract:
The Ras small GTPase functions as a signaling node and is activated by extracellular stimuli. Upon activation Ras interacts with a spectrum of functionallydiverse downstream effectors and stimulates multiplecytoplasmic signaling cascades that regulat

Materials & Methods:
To confirm Rain localization at the Golgi 1 105 COS-1 cells were plated onto 35-mm MatTek dishes containing number 1.5 glass slides on the bottom (MatTek Corp. Ashland MA)

Microscopic Technique
Fluorescence Microscopy, Immunofluorescence

Cell Type(s)
COS-1