Technical Reference #803

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
MatTek dishes (with a glass coverslip at the bottom) precoated with collagen

803.

Phosphorylation Of Paxillin By P38Mapk Is Involved In The Neurite Extension Of Pc-12 Cells Cai Huang; Christoph H. Borchers; ; Michael D. Schaller; ;;4 and Ken Jacobson, University of North Carolina, The Journal of Cell Biology, 164(803), (2004)
Link To Paper

Abstract:
ell adhesions play an important role in neurite extension. Paxillin a focal adhesion adaptor protein involved in focal adhesion dynamics has beendemonstrated to be required for neurite outgrowth. Howeverthe molecular mechanism by which paxillin reg

Keywords:
neurons; nerve growth factor; focal adhesions; neurite outgrowth; mass spectrometry

Materials & Methods:
Cells stably expressing EGFP-paxillin or EGFP-paxS85A were plated on collagen-coated MatTek dishes and treated with 100 ng/ml NGF for 36 h. TIRF and epifluorescence images were taken as described in Materials and methods. (

Microscopic Technique
Fluorescence Microscopy, Epifluorescence Microscopy, TIRF

Cell Type(s)
PC12