Technical Reference #800

800.

Flagellar Motility Is Critical For Listeria Monocytogenes Biofilm Formation Katherine P. Lemon; Darren E. Higgins; and Roberto Kolter, Children's Hospital Boston, The Journal of Bacteriology, 189(800), (2007)
Link To Paper

Abstract:
The food-borne pathogen Listeria monocytogenes attaches to environmental surfaces and forms biofilms that can be a source of food contamination yet little is known about the molecular mechanisms of its biofilmdevelopment. We observed that nonmotile mut

Materials & Methods:
We confirmed that the overnight culture of the wild type and nonglycosylated-flagellum mutant were motile by microscopy. Dishes (P35G-1.5-14-C; MatTek Co. Ashland MA) were inoculated with 1.5 ml of each culture at time zero.

Microscopic Technique
Light Microscopy

Cell Type(s)
M35303A