Technical Reference #799

799.

In Situ Fluorescence Analysis Demonstrates Active Sirna Exclusion From The Nucleus By Exportin 5 Thomas Ohrt; Dennis Merkle; Karin Birkenfeld; Christophe J. Echeverri and Petra Schwille, Dresden University of Technology, Nucleic Acids Research, 34(799), (2006)
Link To Paper

Abstract:
Two types of short double-stranded RNA molecules namely microRNAs (miRNAs) and short interfering RNAs (siRNAs) have emerged recently as importantregulators of gene expression. Although these moleculesshow similar sizes and structural features the

Materials & Methods:
For 6-well plates and MatTek chambers (MatTek Corporation USA) cells were sown one day before transfection at a density of 1.5–3 · 105 cells/well in antibiotic-free media.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HeLa