Technical Reference #798

798.

Global Analysis Of Candidate Genes Important For Fitness In A Competitive Biofilm Using Dna-Array-Based Transposon Mapping Lauren M. Junker; Joseph E. Peters and Anthony G. Hay, Cornell University, Microbiology, 152(798), (2006)
Link To Paper

Abstract:
Escherichia coli strain PHL628 was subjected to saturating Tn5 transposon mutagenesis and then grown under competitive planktonic or biofilm conditions. The locations of transposon insertionsfrom the remaining cells were then mapped on a gene array. The

Materials & Methods:
As biofilm attachment may be impacted by surface chemistry biofilm development on glass was also examined in pulsed-batch culture (see below) using untreated glass-bottomed culture dishes (MatTek) which provide a hydrophilic surface.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
E. Coli