Technical Reference #796

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
MatTek dishes (Ashland; MA)

796.

Naked2 Acts As A Cargo Recognition And Targeting Protein To Ensure Proper Delivery And Fusion Of Tgf-–Containing Exocytic Vesicles At The Lower Lateral Membrane Of Polarized Mdck Cells Cunxi Li;* Mingming Hao; Zheng Cao;* Wei Ding;* Ramona Graves-Deal;* Jianyong Hu;* David W. Piston; and Robert J. Coffey, Vanderbilt University, Molecular Biology of the Cell, 18(796), (2007)
Link To Paper

Abstract:
Transforming growth factor- (TGF-) is the major autocrine EGF receptor ligand in vivo. In polarized epithelial cellsproTGF- is synthesized and then delivered to the basolateral cell surface. We previously reported that Naked2 interactswith basolatera

Materials & Methods:
(EGFP)- tagged Naked2 vesicles and possible fusion events between vesicle–vesicle and vesicle–plasma membrane on MatTek plates.

Microscopic Technique
Confocal Microscopy, Time Lapse

Cell Type(s)
MDCK, Naked2