Technical Reference #791

791.

Regulation Of Nuclear Translocation Of Forkhead Transcription Factor Afx By Protein Kinase B Hiroshi Takaishi*; Hiroaki Konishi*; Hidenori Matsuzaki*; Yoshitaka Ono; Yasuhito Shirai*; Naoaki Saito*; Tadahiro Kitamura‡; Wataru Ogawa‡; Masato Kasuga‡; Ushio Kikkawa*§; and Yasutomi Nishizuka, Kobe University, PNAS, 96(791), (1999)
Link To Paper

Abstract:
The regulation of intracellular localization of AFX a human Forkhead transcription factor was studied. AFX was recovered as a phosphoproteinfrom transfected COS-7 cells growing in the presence of FBSand the phosphorylation was eliminated by wortman

Materials & Methods:
CHO-K1 cells transfected with AFX-GFP or its mutants were spread onto glass-bottom culture dishes (MatTek Ashland MA) and maintained for at least 16 h before observation.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
CHO-K1