Technical Reference #786

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm glass-bottomed dishes (Mat-Tek; Ashland;MA) six-well plates or 35-mm dishes with coverslips

786.

Orphan G Protein-Coupled Receptors Mrga1 And Mrgc11 Are Distinctively Activated By Rf-Amide-Related Peptides Through The Gq/11 Pathway Sang-Kyou Han*; Xinzhong Dong‡; Jong-Ik Hwang*; Mark J. Zylka‡; David J. Anderson‡; and Melvin I. Simon, California Institute of Technology, PNAS, 99(786), (2002)
Link To Paper

Abstract:
MrgA1 and MrgC11 belong to a recently identified family of orphan G-protein coupled receptors called mrgs (mas-related genes). Theyare only expressed in a specific subset of sensory neurons that areknown to detect painful stimuli. However the precis

Materials & Methods:
MrgA1-GFP or MrgC11-GFP stably expressing HEK293 cells were grown in 35-mm glass-bottomed dishes (Mat- Tek AshlandMA) inDMEMwith 10% FBS.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEK-293