Technical Reference #785

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
culture dishes equipped with glass bottoms for direct imaging (MatTek; Loveland; OH)

785.

Nuclear Localization Of Pyrrole–Imidazole Polyamide–Fluorescein Conjugates In Cell Culture Timothy P. Best*; Benjamin S. Edelson*; Nicholas G. Nickols; and Peter B. Dervan, California Institute of Technology, PNAS, 100(785), (2003)
Link To Paper

Abstract:
A series of hairpin pyrrole–imidazole polyamide–fluorescein conjugates were synthesized and assayed for cellular localization.Thirteen cell lines representing 11 human cancers one humantransformed kidney cell line and one murine leukemia cell line

Materials & Methods:
Incubations were performed by adding 150 l of cells into culture dishes equipped with glass bottoms for direct imaging (MatTek

Microscopic Technique
Confocal Microscopy

Cell Type(s)
SK-BR-3 cells