Technical Reference #779

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
Mattek 35-mm plates

779.

Nav1.5 E1053K Mutation Causing Brugada Syndrome Blocks Binding To Ankyrin-G And Expression Of Nav1.5 On The Surface Of Cardiomyocytes Peter J. Mohler*‡; Ilaria Rivolta§; Carlo Napolitano§; Guy LeMaillet; Stephen Lambert; Silvia G. Priori§; and Vann Bennett, Duke University, PNAS, 101(779), (2004)
Link To Paper

Abstract:
We identify a human mutation (E1053K) in the ankyrin-binding motif of Nav1.5 that is associated with Brugada syndrome a fatalcardiac arrhythmia caused by altered function of Nav1.5. TheE1053K mutation abolishes binding of Nav1.5 to ankyrin-G andal

Keywords:
arrhythmia SCN5A targeting trafficking sudden cardiac death

Materials & Methods:
After washes buffer was removed and the pellet was resuspended in Vectashield and plated onto Mattek 35-mm plates.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Cardiac myocytes