Technical Reference #776

776.

The Metal-Ion-Dependent Adhesion Site In The Von Willebrand Factor-A Domain Of A28 Subunits Is Key To Trafficking Voltage-Gated Ca2 Channels C. Cantı´*; M. Nieto-Rostro*; I. Foucault*; F. Heblich*; J. Wratten; M. W. Richards; J. Hendrich; L. Douglas; K. M. Page; A. Davies; and A. C. Dolphin, University College London, PNAS, 102(776), (2005)
Link To Paper

Abstract:
All auxiliary 2 subunits of voltage-gated Ca2 (CaV) channels contain an extracellular Von Willebrand factor-A (VWA) domainthat in 2-1 and -2 has a perfect metal-ion-dependent adhesionsite (MIDAS). Modeling of the 2-2 VWA domain shows it to behi

Keywords:
integrin neuron motif expression

Materials & Methods:
For imaging GFP in live cells glass-bottomed dishes (MatTek Ashland MA) were mounted in a humidified chamber on the microscope and perfused with 5% CO2.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
COS-7