Technical Reference #775

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottom dishes (MatTek; Ashland; MA)

775.

Real-Time Imaging Of Type Iii Secretion: Salmonella Sipa Injection Into Host Cells Markus C. Schlumberger; Andreas J. Mu¨ ller; Kristin Ehrbar; Brit Winnen; Iwan Duss; Ba¨ rbel Stecher; and Wolf-Dietrich Hardt, Eidgeno¨ ssische Technische Hochschule, PNAS, 102(775), (2005)
Link To Paper

Abstract:
Many pathogenic and symbiotic Gram-negative bacteria employ type III secretion systems to inject ‘‘effector’’ proteins into eukaryotichost cells. These effectors manipulate signaling pathways toinitiate symbiosis or disease. By using time-lapse micros

Keywords:
effector protein time-lapse microscopy

Materials & Methods:
pGFP–InvB-transfected Cos7 cells on glass-bottom dishes (MatTek Ashland MA) were transferred into Hank’s balanced salt solution (GIBCO)

Microscopic Technique
Confocal Microscopy

Cell Type(s)
COS-7