Technical Reference #772

772.

Beyond Rayleigh’S Criterion: A Resolution Measure With Application To Single-Molecule Microscopy Sripad Ram*; E. Sally Ward*; and Raimund J. Ober, University of Texas Dallas, PNAS, 103(772), (2006)
Link To Paper

Abstract:
Rayleigh’s criterion is extensively used in optical microscopy for determining the resolution of microscopes. This criterion imposesa resolution limit that has long been held as an impediment forstudying nanoscale biological phenomenon through an opti

Keywords:
Cramer-Rao lower bound photon statistics Fisher information matrix fluorescence microscopy

Materials & Methods:
To image single DNA molecules a cleaned glass-bottomed dish (MatTek Ashland MA) was coated with a layer of polylysine (0.01% solution)

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
DNA molecules