Technical Reference #769

769.

Redox Sensor Ctbp Mediates Hypoxia-Induced Tumor Cell Migration Qinghong Zhang*; Su-Yan Wang*; Amanda C. Nottke*; Jonathan V. Rocheleau‡; David W. Piston‡; and Richard H. Goodman, Oregon Health and Sciences University, PNAS, 103(769), (2006)
Link To Paper

Abstract:
The rapid growth and poor vascularization of solid tumors expose cancer cells to hypoxia which promotes the metastatic phenotypeby reducing intercellular adhesion and increasing cell motility andinvasiveness. In this study we found that hypoxia incr

Keywords:
NADH E-cadherin metastasis HIF1 adhesion

Materials & Methods:
Cells were grown for 2 days on glass 35-mm dishes (MatTek Ashland 9032 www.pnas.orgcgidoi10.1073pnas.0603269103 Zhang et al. MA) and maintained at 37°C and 5% CO2 in a humidified chamber on the microscope stage (Zeiss).

Microscopic Technique
Confocal Microscopy

Cell Type(s)
H1299, HT29