Technical Reference #767

767.

Knockin Mice Expressing Fluorescent Delta-Opioid Receptors Uncover G Protein-Coupled Receptor Dynamics In Vivo Grégory Scherrer*; ; Petra Tryoen-Tóth*; ; Dominique Filliol*; Audrey Matifas*; Delphine Laustriat*; Yu Q. Cao ; Allan I. Basbaum ; Andrée Dierich*; Jean-Luc Vonesh*; Claire Gavériaux-Ruff*; and Brigitte L. Kieffer*;, Centre National de la Recherche Scientifique/Institut National de la Santé et de la Recherche Médicale/Université Louis Pasteur Stanford University School of Medicine University of California San Francisco, PNAS, 103(767), (2006)
Link To Paper

Abstract:
The combination of fluorescent genetically encoded proteins with mouse engineering provides a fascinating means to studydynamic biological processes inmammals. At present greenfluorescent protein (GFP) mice were mainly developed to study gene expre

Keywords:
behavioral desensitization | green fluorescent protein | neuroanatomy | real-time imaging | receptor internalization

Materials & Methods:
Cells were seeded in glass-bottom 32-mm diameter plastic dishes (MatTek) coated with polyL-lysine (Sigma).

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Neurons - Hippocampal