Technical Reference #762

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
six-well plates or 35-mm dishes with coverslips (MatTek Corp.; Ashland;MA)

762.

Neuronal Growth And Survival Mediated By Eif5A; A Polyamine-Modified Translation Initiation Factor Yunfei Huang*; Daniel S. Higginson*; Lynda Hester*; Myung Hee Park; and Solomon H. Snyder, Johns Hopkins University School of Medicine, PNAS, 104(762), (2007)
Link To Paper

Abstract:
Eukaryotic translation initiation factor 5A (eIF5A) the only known protein containing the polyamine-derived amino acid hypusinemodulates protein synthesis. We show that neurotrophic andneuroprotective actions of nerve growth factor (NGF) are mediate

Keywords:
hypusine spermidine nerve growth factor arginase deoxyhypusine synthase

Materials & Methods:
An aliquot of NGF-primed cells was washed once in serum-free DMEM resuspended in serum-free medium and plated on collagen IV-coated six-well plates or 35-mm dishes with coverslips (MatTek Corp. AshlandMA).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
PC12