Technical Reference #761

761.

Microtubule Function In Fibroblast Spreading Is Modulated According To The Tension State Of Cell–Matrix Interactions Sangmyung Rhee; Hongmei Jiang; Chin-Han Ho; and Frederick Grinnell, University of Texas Southwestern Medical Center, PNAS, 104(761), (2007)
Link To Paper

Abstract:
Mechanical and physical features of the extracellular environment dramatically impact cell shape. Fibroblasts interacting with 3Drelaxed collagen matrices appear much different from cells on 2Dcollagen-coated surfaces and form dendritic cell extension

Keywords:
adhesion cell plasticity cytoskeleton extracellular matrix mechanosignaling

Materials & Methods:
Time-lapse microscopy was carried out with glass bottom culture dishes (MatTek Ashland MA) placed in a 37°C environment chamber.

Microscopic Technique
Time Lapse Microscopy

Cell Type(s)
HFF