Technical Reference #760

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm glass-bottomed dishes (MatTek Corporation)

760.

Enhanced Egfp-Chromophore-Assisted Laser Inactivation Using Deficient Cells Rescued With Functional Egfp-Fusion Proteins Eric A. Vitriol*‡; Andrea C. Uetrecht*; Feimo Shen‡; Ken Jacobson*§; and James E. Bear, University of North Carolina Chapel Hill, PNAS, 104(760), (2004)
Link To Paper

Abstract:
Chromophore-assisted laser inactivation (CALI) is a light-mediated technique that offers precise spatiotemporal control of proteininactivation enabling better understanding of the protein’s role incell function. EGFP has been used effectively as a CA

Keywords:
capping protein Mena/VASP spatiotemporal lentivirus

Materials & Methods:
For live-cell experiments Rat2 cells were plated onto 35-mm glass-bottomed dishes (MatTek Corporation) coated with 10 mg/ml fibronectin (Sigma).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Rat2