Technical Reference #758

758.

Mechanisms Of Host Cell Exit By The Intracellular Bacterium Chlamydia Kevin Hybiske and Richard S. Stephens*, University of California Berkeley, PNAS, 104(758), (2007)
Link To Paper

Abstract:
The mechanisms that mediate the release of intracellular bacteria from cells are poorly understood particularly for those that livewithin a cellular vacuole. The release pathway of the obligateintracellular bacterium Chlamydia from cells is unknown.

Keywords:
extrusion lysis release

Materials & Methods:
HeLa 229 cells were plated on glass bottom culture dishes (MatTek Ashland MA) or 24-well plates and typically infected with Chlamydia diluted in Hanks’s balanced salt solution

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HeLa