Technical Reference #757

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm plates with glass coverslips (MatTek; Ashland; MA)

757.

Otopetrin 1 Activation By Purinergic Nucleotides Regulates Intracellular Calcium Inna Hughes*; Mitsuyoshi Saito; Paul H. Schlesinger; and David M. Ornitz, Washington University, PNAS, 104(757), (2007)
Link To Paper

Abstract:
Otopetrin1 (Otop1) is a multitransmembrane domain protein required for the formation of otoconia in the vertebrate inner ear.Otoconia are complex calcium carbonate (CaCO3) biominerals thatare required for the sensation of gravity. Examination of the

Keywords:
biomineralization otoconia purinergic receptor

Materials & Methods:
Cells were plated on 35-mm plates with glass coverslips (MatTek Ashland MA) for 24 h before transfection.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
COS-7, ROS 17/2.8, HEK-293