Technical Reference #754

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm glass-bottom dishes (MatTek; Ashland; MD)

754.

Physical Plasticity Of The Nucleus In Stem Cell Differentiation J. David Pajerowski*; Kris Noel Dahl; Franklin L. Zhong*; Paul J. Sammak‡; and Dennis E. Discher, University of Pennsylvania, PNAS, 104(754), (2007)
Link To Paper

Abstract:
Cell differentiation in embryogenesis involves extensive changes in gene expression structural reorganization within the nucleusincluding chromatin condensation and nucleoprotein immobilization.We hypothesized that nuclei in naive stem cells wouldt

Keywords:
chromatin remodeling cell mechanics nuclear plasticity

Materials & Methods:
TC7 cells plated on 35-mm glass-bottom dishes (MatTek Ashland MD) were transfected with the GFP constructs using Lipofectamine 2000 (LF2k) according to the manufacturer's instructions

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
TC7