Technical Reference #753

753.

Proinsulin Maturation; Misfolding; And Proteotoxicity Ming Liu*; Israel Hodish*; Christopher J. Rhodes; and Peter Arvan, University of Michigan, PNAS, 104(753), (2007)
Link To Paper

Abstract:
As a tool to explore proinsulin (PI) trafficking a human PI cDNA has been constructed with GFP fused within the C peptide. In regulatedsecretory cells containing appropriate prohormone convertases thehProCpepGFP construct undergoes endoproteolytic p

Keywords:
diabetes mellitus endoplasmic reticulum storage disease insulin secretion proinsulin disulfide isomers

Materials & Methods:
INS832/13 cells (26) were plated onto 35-mm gridded glass-bottom culture dishes (Mat- Tek) at 30% confluence 1 d before transfection.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
INS832/13