Technical Reference #751

751.

Force Fluctuations And Polymerization Dynamics Of Intracellular Microtubules Clifford P. Brangwynne‡; F. C. MacKintosh§; and David A. Weitz, Harvard University, PNAS, 104(751), (2007)
Link To Paper

Abstract:
Microtubules are highly dynamic biopolymer filaments involved in a wide variety of biological processes including cell divisionmigration and intracellular transport. Microtubules are very rigidand form a stiff structural scaffold that resists deform

Keywords:
cytoskeleton mechanics nonequilibrium persistence length rigidity

Materials & Methods:
Cells were sparsely plated onto glass-bottomed 35-mm dishes (MatTek) and allowed to adhere and spread overnight.

Microscopic Technique
Fluorescence Microscopy, Epifluorescence Microscopy

Cell Type(s)
COS-7