Technical Reference #750

750.

A Role For Ib Kinase 2 In Bipolar Spindle Assembly Jeffrey T. Irelan*; Thomas J. Murphy*; Paul D. DeJesus*; Hsiangling Teo; DingYue Xu; Maria A. Gomez-Ferreria‡; Yingyao Zhou*; Loren J. Miraglia*; Daniel R. Rines*; Inder M. Verma§; David J. Sharp‡; Vinay Tergaonkar§; and Sumit K. Chanda, Genomics Institute of the Novartis Research Foundation, PNAS, 104(750), (2007)
Link To Paper

Abstract:
IB kinase 2 (IKK2 or IKK) is a component of the IKK complex that coordinates the cellular response to a diverse set of extracellularstimuli including cytokines microbial infection and stress. Inresponse to an external stimulus the complex is activ

Keywords:
Aurora A mitosis NF-B spindle polarity

Materials & Methods:
HeLa cells expressing GFP-a-tubulin where reverse-transfected with siRNA IKK2 no. 2. Briefly 80 nmol of the siRNA was diluted in 500 ml of OptiMem (Invitrogen) and placed in a 35-mm glass bottom dish (MatTek).

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HeLa