Technical Reference #749

749.

A Role For The Inositol Kinase Ipk1 In Ciliary Beating And Length Maintenance Bhaskarjyoti Sarmah*; Virginia P. Winfrey*; Gary E. Olson*; Bruce Appel; and Susan R. Wente, Washington University, PNAS, 104(749), (2007)
Link To Paper

Abstract:
Cilia project from cells as membranous extensions with microtubule structural cores assembling from basal bodies by intraflagellartransport (IFT). Here we report a ciliary role for the inositol13456-pentakisphosphate 2-kinase (Ipk1) that generat

Keywords:
cilia inositol hexakisphosphate kidney development left–right asymmetry zebrafish

Materials & Methods:
ive embryos (12 hpf) were removed from their chorions mounted in SeaPlaque low-melting agarose (Biowhittaker Molecular Applications) (1.0% in embryo medium) in microwells of glass-bottom culture dishes (MatTek)

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Cilia