Technical Reference #748

748.

Ubiquitin-Dependent Virus Particle Budding Without Viral Protein Ubiquitination Maria Zhadina*; Myra O. McClure; Marc C. Johnson‡; and Paul D. Bieniasz, The Rockefeller University, PNAS, 104(748), (2007)
Link To Paper

Abstract:
An essential step in the release of an extracellular enveloped virus particle is a budding event that ultimately separates virion andhost cell membranes. For many enveloped viruses membranefission requires the recruitment of the class E vacuolar prot

Materials & Methods:
HeLa cells (4 105) on 35-mm poly-Dlysine- coated glass bottom dishes (Mattek) were cotransfected with plasmids expressing CFP-fused and unfused Lck–Gag at a 1:1 ratio either alone or along with 200 ng of pCR3.1/YFP–WWP1–WW.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HeLa