Technical Reference #745

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottom dishes (P35G-1.5-14-C; MatTek)

745.

Snarepin/Munc18 Promotes Adhesion And Fusion Of Large Vesicles To Giant Membranes David Tareste; Jingshi Shen; Thomas J. Melia; and James E. Rothman, Columbia University, PNAS, 105(745), (2008)
Link To Paper

Abstract:
Exocytic vesicle fusion requires both the SNARE family of fusion proteins and a closely associated regulatory subunit of the Sec1/Munc18 (SM) family. In principle SM proteins could act at an earlySNARE assembly step to promote vesicle–plasma membrane

Keywords:
giant liposomes Sec1/Munc18

Materials & Methods:
Samples were prepared in glass-bottom dishes (P35G-1.5-14-C; MatTek) coated with 10% (wt/vol)BSAfor1hatroomtemperature

Microscopic Technique
Confocal Microscopy

Cell Type(s)
E. Coli