Technical Reference #744

744.

Identification Of Sites For Exponential Translation In Living Dendrites Christy Job and James Eberwine*, University of Pennsylvania, The Journal of Cell Biology, 98(744), (2001)
Link To Paper

Abstract:
Neuronal processes contain mRNAs and membrane structures and some forms of synaptic plasticity seem to require protein synthesisin dendrites of hippocampal neurons. To quantitate dendriticprotein synthesis we used multiphoton microscopy of green flu

Materials & Methods:
Primary cultured hippocampal neurons were prepared from embryonic day 18 rats and grown at low density on CELLocate coverslips (Eppendorf no. 5245 953.005) embedded into 3-mm dishes by MatTek Ashland MA (no. P35G-1.5-7-C-grid).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Neurons - Hippocampal