Technical Reference #743

743.

Defective Presentation Of The Cd1D1-Restricted Natural Va14Ja18 Nkt Lymphocyte Antigen Caused By -D-Glucosylceramide Synthase Deficiency Aleksandar K. Stanic*; A. Dharshan De Silva*; Jang-June Park*; Venkataraman Sriram‡§; Shinichi Ichikawa; Yoshio Hirabyashi; Kyoko Hayakawa; Luc Van Kaer*; Randy R. Brutkiewicz‡§; and Sebastian Joyce, Vanderbilt University, PNAS, 100(743), (2003)
Link To Paper

Abstract:
Va14Ja18 natural T (NKT) cells play an immunoregulatory role which is controlled by a self glycolipid(s) presented by CD1d.Although the synthetic antigen -D-galactosylceramide (-D-Gal-Cer) stimulates all Va14Ja18 NKT cells -anomeric D-glycosylcer

Materials & Methods:
Cells (5 106) were plated in glass-bottom microwells (MatTek) and incubated overnight at 37°C to allow the cells to adhere to the wells.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Natural killer T