Technical Reference #742

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
gridded glass coverslip bottom culture dishes (MatTek; Ashland;MA)

742.

Uniform Polarity Microtubule Assemblies Imaged In Native Brain Tissue By Second-Harmonic Generation Microscopy Daniel A. Dombeck*; Karl A. Kasischke*; Harshad D. Vishwasrao*; Martin Ingelsson; Bradley T. Hyman; and Watt W. Webb, Cornell University, PNAS, 100(742), (2003)
Link To Paper

Abstract:
Microtubule (MT) ensemble polarity is a diagnostic determinant of the structure and function of neuronal processes. Here polarizedMT structures are selectively imaged with second-harmonic generation(SHG) microscopy in native brain tissue. This SHG is

Materials & Methods:
Embryonic day 18 rat hippocampi obtained from BrainBits (South Illinois University School of Medicine Springfield) were dissociated and plated in gridded glass coverslip bottom culture dishes (MatTek AshlandMA)

Microscopic Technique
Fluorescence Microscopy, Immunofluorescence

Cell Type(s)
Neurons - Hippocampal