Technical Reference #741

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
collagen-coated 35-mm plastic dishes with glass bottoms (MatTek; Ashland; MA)

741.

Independent -Arrestin 2 And G Protein-Mediated Pathways For Angiotensin Ii Activation Of Extracellular Signal-Regulated Kinases 1 And 2 Huijun Wei*; Seungkirl Ahn*; Sudha K. Shenoy*; Sadashiva S. Karnik‡; La´ szlo´ Hunyady§; Louis M. Luttrell; and Robert J. Lefkowitz*, Duke University, PNAS, 100(741), (2003)
Link To Paper

Abstract:
Stimulation of a mutant angiotensin type 1A receptor (DRY AAY) with angiotensin II (Ang II) or of a wild-type receptor with an AngII analog ([sarcosine1Ile4Ile8]Ang II) fails to activate classical heterotrimericG protein signaling but does lead to r

Materials & Methods:
One day after transfection cells were split onto collagen-coated 35-mm plastic dishes with glass bottoms (MatTek Ashland MA) and cultured overnight at 37°C.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEK-293