Technical Reference #740

740.

Imaging Tetrahymena Ribozyme Splicing Activity In Single Live Mammalian Cells Sumitaka Hasegawa*; W. Coyt Jackson; Roger Y. Tsien; and Jianghong Rao, University of California Los Angeles (UCLA), PNAS, 100(740), (2003)
Link To Paper

Abstract:
Tetrahymena ribozymes hold promise for repairing genetic disorders but are largely limited by their modest splicing efficiency andlow production of final therapeutic proteins. Ribozyme evolutionin intact living mammalian cells would greatly facilitate

Materials & Methods:
Twenty-four hours after transfection cells were reseeded onto a 35-mm glass-bottom culture dish (MatTek Ashland MA).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
COS-1