Technical Reference #738

738.

Requirement For Arf6 In Breast Cancer Invasive Activities Shigeru Hashimoto*; Yasuhito Onodera*; Ari Hashimoto*; Miwa Tanaka‡; Michinari Hamaguchi‡; Atsuko Yamada*; and Hisataka Sabe, Osaka Bioscience Institute, PNAS, 101(738), (2004)
Link To Paper

Abstract:
In most human breast cancer cell lines there is a direct correlation between their in vivo invasive phenotypes and in vitro invasionactivities. Here we found that ADP-ribosylation factor 6 (Arf6) islocalized at the invadopodia of the cultured breast

Materials & Methods:
Coating of glass-bottomed dishes (MatTek Ashland MA) with the fluorescence-conjugated gelatin and the subsequent crosslinking of the gelatin with 0.5% glutaraldehyde were performed as described (12).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
MDA-MB-231