Technical Reference #737

737.

In Vivo Dynamics Of Chromatin-Associated Complex Formation In Mammalian Nucleotide Excision Repair Martijn J. Mone´ *; Tytus Bernas; Christoffel Dinant*; Feliks A. Goedvree*; Erik M. M. Manders*; Marcel Volker‡; Adriaan B. Houtsmuller§; Jan H. J. Hoeijmakers; Wim Vermeulen; and Roel van Driel, University of Amsterdam Centre Erasmus Medical Centre, PNAS, 101(737), (2004)
Link To Paper

Abstract:
Chromatin is the substrate for many processes in the cell nucleus including transcription replication and various DNA repair systemsall of which require the formation of multiprotein machinerieson the chromatin fiber. We have analyzed the kinetics

Keywords:
DNA repair ERCC1-XPF in vivo kinetics TFIIH

Materials & Methods:
Cells were cultured in glass-bottomed Petri dishes (MatTek Ashland MA) and were used at 60–80% confluency.

Microscopic Technique
Fluorescence Microscopy, Immunofluorescence

Cell Type(s)
CHO