Technical Reference #735

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
gridded coverslips (MatTek; Ashland; MA)

735.

Visualization Of Retrovirus Budding With Correlated Light And Electron Microscopy Daniel R. Larson*‡; Marc C. Johnson§; Watt W. Webb*; and Volker M. Vogt, Cornell University, PNAS, 102(735), (2005)
Link To Paper

Abstract:
We have used correlated scanning EM (SEM) and multiphotonfluorescence microscopy to visualize budding of virus-like particles(VLPs) of Rous sarcoma virus (RSV) and HIV type 1 (HIV-1).

Keywords:
assembly Gag multiphoton fluorescence tracking

Materials & Methods:
For correlative experiments cells were plated onto gridded coverslips (MatTek Ashland MA) before transfection and viewed 24–48 h posttransfection.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
DF-1