Technical Reference #733

733.

Spatiotemporal Control Of Spindle Midzone Formation By Prc1 In Human Cells Changjun Zhu; Eric Lau; Robert Schwarzenbacher; Ella Bossy-Wetzel; and Wei Jiang, Burnham Institute for Medical Research, PNAS, 106(733), (2006)
Link To Paper

Abstract:
We have examined the role of PRC1 a midzone-associated microtubule bundling Cdk substrate protein in regulating the spatiotemporalformation of the midzone in HeLa cells. Cdk-mediatedphosphorylation of PRC1 in early mitosis holds PRC1 in an inactive

Keywords:
Cdk phosphorylation mitosis cytokinesis microtubule-associated proteins microtubule bundling

Materials & Methods:
HeLa cells grown on a 35-mm polylysinecoated glass-bottom microwell dish (MatTek Ashland MA) were transfected with mammalian expression plasmids andor siRNA by using Lipofectamine 2000 or Oligofectamine (Invitrogen).

Microscopic Technique
Time Lapse Microscopy

Cell Type(s)
HeLa