Technical Reference #730

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm glass-bottomed dishes

730.

Linkage Between Cellular Communications; Energy Utilization; And Proliferation In Metastatic Neuroendocrine Cancers Joseph E. Ippolito*; Matthew E. Merritt; Fredrik Ba¨ ckhed*; Krista L. Moulder‡; Steven Mennerick‡§; Jill K. Manchester*; Seth T. Gammon; David Piwnica-Worms; and Jeffrey I. Gordon, University of Texas Southwestern Medical Center, PNAS, 103(730), (2006)
Link To Paper

Abstract:
To identify metabolic features that support the aggressive behavior of human neuroendocrine (NE) cancers we examined metastaticprostate NE tumors and derived prostate NE cancer (PNEC)cell lines from a transgenic mouse model using a combination ofmagic

Keywords:
metabolome-directed cancer therapy electrophysiology GABA signaling and shunt magic angle spinning-NMR metabolomics

Materials & Methods:
PNEC cells (2 ´ 106 cells) were plated on poly(L-lysine)- and laminin-coated 35-mm glass-bottom dishes (Mattek Homer MA) and grown in NPBM media containing 10% FCS for 48 h.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
PNEC