Technical Reference #728

728.

Differential Regulation Of Phagosome Maturation In Macrophages And Dendritic Cells Mediated By Rhogtpases And Ezrin–Radixin–Moesin (Erm) Proteins Lars-Peter Erwig*‡; Kathleen A. McPhilips*; Murray W. Wynes*; Alexander Ivetic§; Anne J. Ridley§; and Peter M. Henson*, National Jewish Medical and Research Center, PNAS, 103(728), (2006)
Link To Paper

Abstract:
Deletion of apoptotic cells from tissues involves their phagocytosis by macrophages dendritic cells and tissue cells. Although muchattention has been focused on the participating ligands receptorsand mechanisms of uptake little is known of the disp

Materials & Methods:
Cells were plated in 35-mm glass-bottom dishes (MatTek Ashland MA) and matured by culturing in medium containing IL-4 andM colony-stimulating factor (R & D Systems Minneapolis MN) as described (32).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HMDDC