Technical Reference #726

726.

Metabolic Regulation Of Sirt1 Transcription Via A Hic1:Ctbp Corepressor Complex Qinghong Zhang*; Su-Yan Wang*; Capucine Fleuriel‡; Dominique Leprince‡; Jonathan V. Rocheleau§; David W. Piston§; and Richard H. Goodman*, Oregon Health and Sciences University, PNAS, 104(726), (2007)
Link To Paper

Abstract:
The Sir2 histone deacetylases are important for gene regulationmetabolism and longevity. A unique feature of these enzymes istheir utilization of NAD as a cosubstrate which has led to thesuggestion that Sir2 activity reflects the cellular energy st

Keywords:
redox; NAD; NADH

Materials & Methods:
Cells were grown for 2 days on 35-mm glass dishes (MatTek Ashland MA) maintained at 37°C and 5% CO2 in a humidified chamber on the microscope stage (Carl Zeiss Thornwood NY).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
H1299