Technical Reference #725

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
PLL-coated glass bottom Mattek dishes

725.

Enhanced Sensitivity To Igf-Ii Signaling Links Loss Of Imprinting Of Igf2 To Increased Cell Proliferation And Tumor Risk Atsushi Kaneda*; Chiaochun J. Wang; Raymond Cheong; Winston Timp*; Patrick Onyango*; Bo Wen*; Christine A. Iacobuzio-Donahue‡; Rolf Ohlsson§; Rita Andraos; Mark A. Pearson; Alexei A. Sharov; Dan L. Longo;, Johns Hopkins University School of Medicine, PNAS, 104(725), (2007)
Link To Paper

Abstract:
Loss of imprinting (LOI) of the insulin-like growth factor-II gene(IGF2) leading to abnormal activation of the normally silent maternalallele is a common human epigenetic population variantassociated with a 5-fold increased frequency of colorectal ne

Keywords:
Akt cancer chemoprevention epigenetics signal transduction

Materials & Methods:
The day prior to transfection cells were trypsinized and seeded into PLL-coated glass bottom Mattek dishes.

Microscopic Technique
Light Microscopy

Cell Type(s)
3T3