Technical Reference #721

721.

Splicing Promotes Rapid And Efficient Mrna Export In Mammalian Cells Patricia Valencia; Anusha P. Dias; and Robin Reed*, Harvard Medical School, PNAS, 105(721), (2008)
Link To Paper

Abstract:
The numerous steps in protein gene expression are extensivelycoupled to one another through complex networks of physical andfunctional interactions. Indeed >25 coupled reactions often reciprocalhave been documented among such steps as transcription

Materials & Methods:
Transiently transfected HeLa cells were plated on fibronectin-coated 35-mm dishes with glass coverslip bottoms (MatTek) 24 h posttransfection.

Microscopic Technique
Microinjection

Cell Type(s)
HeLa