Technical Reference #717

717.

The Microtubule-Associated Protein Tumor Overexpressed Gene/Cytoskeleton-Associated Protein 5 Is Necessary For Myelin Basic Protein Expression In Oligodendrocytes Victor P. Francone; Michael J. Maggipinto; Linda D. Kosturko; and Elisa Barbarese, University of Connecticut, The Journal of Neuroscience, 27(717), (2007)
Link To Paper

Abstract:
Tumor overexpressed gene (TOG) protein encoded by cytoskeleton-associated protein CKAP5 is a microtubule-associated protein thatbinds to heterogeneous nuclear ribonucleoprotein (hnRNP) A2. hnRNP A2 is an RNA trafficking factor that associates with myel

Keywords:
cell culture; differentiation; PCR-based short-hairpin RNA interference; RNA trafficking; translation; fluorescence microscopy

Materials & Methods:
Microinjection of cells plated on glass bottom dishes (MatTek Ashland MA)was performed using an electronic microinjection system (Eppendorf Hamburg Germany).

Microscopic Technique
Confocal Microscopy, Laser Scanning

Cell Type(s)
B104