Technical Reference #712

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
Collagen- or poly-Dlysine- coated 14-mm glass bottom culture dishes (MatTek Corp.; Ashland; MA)

712.

Different Domains Of The Ampa Receptor Direct Stargazin-Mediated Trafficking And Stargazin-Mediated Modulation Of Kinetics Matthew A. Bedoukian; Autumn M. Weeks; and Kathryn M. Partin, Colorado State University, The Journal of Biological Chemistry, 281(712), (2006)
Link To Paper

Abstract:
Stargazin is an accessory protein of AMPA receptors thatenhances surface expression and also affects the biophysicalproperties of the receptor. AMPA receptor domains necessaryfor either of these two processes have not yet been identified.Here we used

Materials & Methods:
Collagen- or poly-Dlysine- coated 14-mm glass bottom culture dishes (MatTek Corp. Ashland MA) were incubated with ECL Attachment Matrix (Upstate Cell Signaling Solutions Lake Placid NY) for 1 h at 37 °C then washed with cMEM before plating cells.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEK-293