Technical Reference #710

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
collagen coated glass bottom culture Petri dishes (MatTek, MA, USA)

710.

In Vitro Assays For Evaluating The Ultraviolet B-Induced Damage In Cultured Human Retinal Pigment Epithelial Cells Hyun-Yi Youn, Vladimir Bantseev, Niels C. Bols, Anthony P. Cullen, Jacob G. Sivak - Not in ACT, University of Waterloo, Journal of Photochemistry and Photobiology, 88(710), 23, (2007)
Link To Paper

Abstract:
The present study demonstrates broadband UV-B-induced damage of cultured human retinal pigment epithelial cells as an effort todevelop an in vitro model that can be used, along with in vivo research and other in vitro efforts, to evaluate the need for re

Keywords:
UVB-induced retinal damage; RPE; Alamar blue assay; Cellular viability; Confocal microscopy; Mitochondrial damage; DNA damage; Phagocytotic activity

Materials & Methods:
In the present study, 1 · 105 cells in 1.5 ml of culture medium were seeded into collagen coated glass bottom culture Petri dishes (MatTek, MA, USA).

Microscopic Technique
Confocal Microscopy

Cell Type(s)
ARPE-19