Technical Reference #708

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
50 mm MatTek glass bottom dishes (MatTek Corp; Ashland; MA) containing photo-etched gridded coverslips (thickness 0.19 - 0.22 mm)

708.

Visual Snapshots Of Intracellular Kinase Activity At The Onset Of Mitosis Zhaohua Dai;; Natalya G. Dulyaninova; Sanjai Kumar; Anne R. Bresnick; and David S. Lawrence, Albert Einstein College of Medicine of Yeshiva University, Chemistry & Biology, 14(708), (2007)

Abstract:
Visual snapshots of intracellular kinase activity can be acquired with exquisite temporal control using a light-activatable (caged) sensor thereby providing a means to interrogate enzymatic activity at any point during the cell division cycle. Robust pro

Keywords:
Intracellular Kinase Activity; Mitosis

Materials & Methods:
PtK2 cells were cultured at 37 °C for 2 - 3 days (70 - 80% confluence) on 50 mm MatTek glass bottom dishes (MatTek Corp Ashland MA) containing photo-etched gridded coverslips (thickness 0.19 - 0.22 mm) in Dulbeco’s MEM with non-essential amino acids and

Microscopic Technique
Live Cell Enyzmology, Immunofluorescence

Cell Type(s)
Ptk2