Technical Reference #704

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottomed polystyrene dishes (35-mm diameter; glass bottom: grade No. 1.5, diameter: 10 mm; Mattek, Ashland, MA)

704.

Specific Adsorption Of Osteopontin And Synthetic Polypetides To Calcium Oxalate Monohydrate Crystals Adam Taller*, Bernd Grohe*§, Kem A. Rogers#, Harvey A. Goldberg* and Graeme K. Hunter*, University of Western Ontario, Biophysical Journal, 93(704), 1769, (2007)
Link To Paper

Abstract:
Protein-crystal interactions are known to be important in biomineralization. To study the physicochemical basis of such interactions, we have developed a technique that combines confocal microscopy of crystals with fluorescence imaging of proteins. In the

Keywords:
Kidney stones, confocal microscopy, fluorescence imaging, growth habit

Materials & Methods:
200-μl aliquots of calcium oxalate solution were added to glass-bottomed polystyrene dishes (35 mm diameter; glass-bottom: grade no. 1.5, diameter: 10 mm; MATTEK CORPORATION, Ashland, MA).

Microscopic Technique
Confocal Microscopy, Fluorescence Microscopy

Cell Type(s)
COM calcium oxalate monohydrate