Technical Reference #704

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottomed polystyrene dishes (35-mm diameter; glass bottom: grade No. 1.5; diameter: 10 mm; Mattek; Ashland; MA)

704.

Specific Adsorption Of Osteopontin And Synthetic Polypetides To Calcium Oxalate Monohydrate Crystals Adam Taller; Bernd Grohe; Kem A. Rogers; Harvey A. Goldberg and Graeme K. Hunter, University of Western Ontario, Biophysical Journal, 93(704), (2007)
Link To Paper

Abstract:
Protein-crystal interactions are known to be important in biomineralization. To study the physicochemical basis of such interactions we have developed a technique that combines confocal microscopy of crystals with fluorescence imaging of proteins. In the

Keywords:
Kidney stones; confocal microscopy; fluorescence imaging; growth habit

Materials & Methods:
200-μl aliquots of calcium oxalate solution were added to glass-bottomed polystyrene dishes (35 mm diameter; glass-bottom: grade no. 1.5 diameter: 10 mm; MATTEK CORPORATION Ashland MA).

Microscopic Technique
Confocal Microscopy, Fluorescence Microscopy

Cell Type(s)
COM calcium oxalate monohydrate