Technical Reference #703

703.

Simple Quantitative Detection Of Mitochondrial Superoxide Production In Live Cells Partha Mukhopadhyay;a Mohanraj Rajesh;a Kashiwaya Yoshihiro;b György Haskó; and Pál Pachera, National Institutes of Health, Biochemical and Biophysical Research Communications, 358(703), (2007)
Link To Paper

Abstract:
Experiments with isolated mitochondria have established that these organelles are pivotalintracellular sources of superoxide in a variety of pathophysiological conditions. Recently a novelfluoroprobe MitoSOX Red was introduced for selective detection o

Keywords:
Superoxide; Mitochondria; Free radicals; MitoSOX; Antimycin A (AntA); Paraquat (PQ); Doxorubicin (DOX); Cardiomyocytes; H9c2; Human coronary artery endothelial cells (HCAECs)

Materials & Methods:
Cells were cultured in 12 well cell culture plates or glass bottom dishes (MatTek Ashland MA) with DMEM (Gibco Invitrogen Carlsbad CA) containing 10% fetal bovine serum (Invitrogen Carlsbad CA) 100 U/ml penicillin and 100 μg/ml streptomycin at 37

Microscopic Technique
Confocal Microscopy

Cell Type(s)
H9c2