Technical Reference #700

700.

Requirement Of Jip Scaffold Proteins For Nmda-Mediated Signal Transduction Norman J. Kennedy; Gilles Martin; Anka G. Ehrhardt; Julie Cavanagh-Kyros; Chia-Yi Kuan; Pasko Rakic;4 Richard A. Flavell;5 Steven N. Treistman; and Roger J. Davis;6, University of Massachusetts, Genes & Development, 21(700), (2007)
Link To Paper

Abstract:
JIP scaffold proteins are implicated in the regulation of protein kinase signal transduction pathways. To testthe physiological role of these scaffold proteins we examined the phenotype of compound mutant mice thatlack expression of JIP proteins. These

Keywords:
JIP; JNK; scaffold protein; signal transduction]

Materials & Methods:
The cells were resuspended in neurobasal medium containing B27 supplements 1% glutamine 1% penicillin/streptomycin 25 mM glucose and 25 mM KCl and seeded in poly-D-lysine/laminin-coated chamber slides (Becton Dickenson) or dishes (MatTek).

Microscopic Technique
Fluorescence Microscopy, Immunofluorescence

Cell Type(s)
Cerebellar