Technical Reference #696

696.

Perilipin A Is Essential For The Translocation Of Hormone-Sensitive Lipase During Lipolytic Activation Carole Sztalryd; Guoheng Xu;Heidi Dorward;John T. Tansey;Juan A. Contreras;Alan R. Kimmel; and Constantine Londos, National Institutes of Health, The Journal of Cell Biology, 161(696), (2003)
Link To Paper

Abstract:
key step in lipolytic activation of adipocytes isthe translocation of hormone-sensitive lipase (HSL)from the cytosol to the surface of the lipid storagedroplet. Adipocytes fromperilipin-null animals have anelevated basal rate of lipolysis compared w

Keywords:
lipolysis; adipocytes; ADRP/adipophilin; HSL; lipid storage droplets

Materials & Methods:
immunofluorescence experiments cells were plated on Petri dishes containing glass bottoms Translocation of HSL in CHO cells was assessed by plating cells on glass bottom dishes equipped with a cell locator grid (MatTek Corporation)

Microscopic Technique
Fluorescence Microscopy, Immunofluorescence

Cell Type(s)
CHO-K1