Technical Reference #694

694.

Neuroendocrine Plasticity In The Anterior Pituitary: Gnrh Mediated Movement In Vitro And In Vivo Amy M. Navratil; J. Gabriel Knoll; Jennifer D. Whitesell; Stuart A. Tobet; Colin M. Clay, University of California San Diego (UCSD), Endocrinology, 148(694), (2007)
Link To Paper

Abstract:
The secretion of luteinizing hormone (LH) is cued by the hypothalamic neuropeptidegonadotropin-releasing hormone (GnRH). Following delivery to the anterior pituitary gland viathe hypothalamic-pituitary portal vasculature GnRH binds to specific high af

Keywords:
GnRH Mediated; Anterior Pituitary; Neuroendocrine Plasticity

Materials & Methods:
Slices intended for use in video microscopy were individually placed on glass-bottomed culture dishes (MatTek Corporation) that had been previously coated in poly-D-lysine and 8 collagen (Vitrogen 100 Cohesion).

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Pituitaries